Nucleotide sequence of Physarum U6 small RNA
نویسندگان
چکیده
SEQUENCE ORIGIN: Small RNA molecule U6 was isolated from the total cellular RNA of vegetative Phvsarnm microplasmodia (1) by preparative polyacrylamide electroshoresis (2,3). The molecule was radiolabeled in vitro to high-specific-activity using [ T>)-pCp and RNA-ligase, and sequenced by direct chemical methods as we previously described (3). In this sequencing process, pseudouridine scores as a uridine, methylcytosine scores as a cytosine, and methyladenosine scores as an adenosine.
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ورودعنوان ژورنال:
- Nucleic acids research
دوره 15 1 شماره
صفحات -
تاریخ انتشار 1987